Rhodamine WT 2.5% Solution for Total Algae Calibration

Rhodamine WT 2.5% Solution for Total Algae Calibration

2.5% Rhodamine WT concentrate for preparing calibration standards for YSI Total Algae sensors. The concentrate is diluted with deionised or distilled water to produce the required standards for calibration of the Chlorophyll, Phycocyanin and Phycoerythrin channels.

Used for preparing a 125 mg/L working solution from the 2.5% concentrate. This working solution is then used to prepare the 625 µg/L standard for Chlorophyll and Phycocyanin calibration and the 25 µg/L standard for Phycoerythrin calibration.

Key features:

• 2.5% Rhodamine WT concentrate

• Suitable for Chlorophyll, Phycocyanin and Phycoerythrin calibration

• Kingscote Chemicals Rhodamine WT

Equipment required to prepare the standards

• 2.5% Rhodamine WT solution

• Three 1 L volumetric flasks

• High-accuracy pipette capable of measuring 5 mL

• High-accuracy pipette capable of measuring 200 µL

• Transfer pipette

• Three labelled amber storage bottles

• Deionised or distilled water

• Appropriate PPE, including gloves, safety glasses and lab coat

Important: The Rhodamine WT concentrate is not a ready-to-use calibration standard. It must be accurately diluted using suitable laboratory equipment to prepare the required calibration solutions. The accuracy of the standard preparation therefore directly affects the accuracy of the sensor calibration.

Total Algae Calibration Service

We also offer a calibration service for YSI Total Algae sensors. For simplicity, time and cost saving you may wish for our trained technicians to calibrate your sensors.